The White Wolves, who defeated 6-3 to group winners Kyrgyz Republic on Saturday, took the lead after two minutes through Mukhammad Zoirov.
But their celebrations were short-lived after Tajikistan’s Muhamamad Rajabov earned a first-half hat-trick for a 3-1 lead at half-time.
Uzbekistan hit the ground running after the restart and pulled one back in the first minute to trail 3-2.
However, Tajikistan maintained their composure and furthered their advantage with goals from Faridun Nematov (37th), Khalid Saburov (39th) and Idris Yorov (35th, 40th) to win 7-3.
Kyrgyz Republic stayed perfect to finish on top, while Tajikistan will face Afghanistan in the Central Zone play-off on Tuesday.
2019 AFC U-20 Futsal Championship Qualifiers
Uzbekistan 2-7 Tajikistan